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Institut für Physiologie und Pathophysiologie

Image Processing

The acquisition of raw image series with a microscope system is usually followed by methods of image processing. On the one hand, the purpose can be an improvement of the image quality, like noise reduction or deconvolution. On the other hand it can be an automated analysis of the experimental data. In our group, the programming work is mainly done with IDL software (Interactive data language).


Model-based noise reduction

Improving the signal-to-noise ratio, e.g. by using wavelet transforms for the detection of calcium sparks from the background noise.


Automated detection and analysis

Example: Development of a software to detect elementary calcium release events and analyze their frequency and morphology.



A point source of light is displayed by the optics of a microscope in a characteristic way that is known as the "point spread function" (PSF). This PSF can be eather measured or calculated for the microscope system and then be used to compute undistorted data from the acquired images. The deconvolved images are clearer and less blurry.


Neue Publikationen


Endothelial progenitor cells accelerate endothelial regeneration in an in vitro model of Shigatoxin-2a-induced injury via soluble growth factors. Am J Physiol Renal Physiol. 2018 Mar 7. doi: 10.1152/ajprenal.00633.2017. [Epub ahead of print]


Astrocytic glutamine synthetase is expressed in the neuronal somatic layers and down-regulated proportionally to neuronal loss in the human epileptic hippocampus. Glia. 2018 May;66(5):920-933. doi: 10.1002/glia.23292. Epub 2018 Jan 19.


Expansion of functional personalized cells with specific transgene combinations. Nat Commun. 2018 Mar 8;9(1):994. doi: 10.1038/s41467-018-03408-4.


Impact of carbonylation on glutathione peroxidase-1 activity in human hyperglycemic endothelial cells. Redox Biol. 2018 Feb 27;16:113-122. doi: 10.1016/j.redox.2018.02.018. [Epub ahead of print]


Respiration-Entrained Brain Rhythms Are Global but Often Overlooked. Trends Neurosci. 2018 Feb 8. pii: S0166-2236(18)30031-6. doi: 10.1016/j.tins.2018.01.007. [Epub ahead of print] Review.


Modulation of glutathione peroxidase activity by age-dependent carbonylation in glomeruli of diabetic mice. J Diabetes Complications. 2018 Feb;32(2):130-138. doi: 10.1016/j.jdiacomp.2017.11.007. Epub 2017 Nov 22.


Bringing European physiologists together. Acta Physiol (Oxf). 2018 Jan 29. doi: 10.1111/apha.13043. [Epub ahead of print]


Role of protein carbonylation in diabetes. J Inherit Metab Dis. 2018 Jan;41(1):29-38. doi: 10.1007/s10545-017-0104-9. Epub 2017 Nov 6.


Sensitive mass spectrometric assay for determination of 15-deoxy-Δ12,14-prostaglandin J2 and its application in human plasma samples of patients with diabetes. Anal Bioanal Chem. 2018 Jan;410(2):521-528. doi: 10.1007/s00216-017-0748-1. Epub 2017 Nov 16.


Hypertension-evoked RhoA activity in vascular smooth muscle cells requires RGS5. FASEB J. 2017 Dec 5. pii: fj.201700384RR. doi: 10.1096/fj.201700384RR. [Epub ahead of print]

Institut für
Physiologie und Pathophysiologie

Universität Heidelberg

Im Neuenheimer Feld 326

69120 Heidelberg

Telefon:+49 6221 54-4035
Telefax:+49 6221 54-4038