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Institute of Physiology and Pathophysiology

Research Areas


Using skeletal muscle and neuronal preparations for the development of highly sensitive methods, we are seeking to expand state of the art technology in physical and biophysical sciences to the application in basic medical research.


The techniques used by our group include microscopic fluorescence imaging techniques such as, e.g., confocal/multiphoton imaging, ratiometric wide field fluorescence microscopy, microscopic laser application such as, e.g., UV-laser microdissection and IR-optical tweezers, patch clamp and several other voltage clamp techniques, mathematical modelling of cellular and subcellular dynamic processes, and basic image processing techniques especially for studying dynamic processes.

With the help of this broad spectrum of sensitive techniques many clinical relevant questions can be studied in its molecular origins. For example the investigations on Duchenne muscular dystrophy are utilizing the patch-clamp method and the various approaches for determining the total intracellular calcium turnover in diseased single muscle fibers.

The effects of volatile anaesthetics on the contractile proteins of skeletal and heart muscles, as well as on the ryanodine receptors can be ideally studied using permeabilised muscle fibres, with the contractile force and the calcium turnover as very sensitive indicators of cellular and subcellular effects of volatile anaesthetics.

The research projects of the group are embedded in a network of national and international cooperations, which further expand the interdisciplinary approaches of our research topics. This includes collaborative projects in molecular biology, biotechnology and physical chemistry.







Recent Publications

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Impact of carbonylation on glutathione peroxidase-1 activity in human hyperglycemic endothelial cells. Redox Biol. 2018 Jun;16:113-122. doi: 10.1016/j.redox.2018.02.018. Epub 2018 Mar 1.

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Respiration-Entrained Brain Rhythms Are Global but Often Overlooked. Trends Neurosci. 2018 Apr;41(4):186-197. doi: 10.1016/j.tins.2018.01.007. Epub 2018 Feb 9.

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Hypertension-evoked RhoA activity in vascular smooth muscle cells requires RGS5. FASEB J. 2018 Apr;32(4):2021-2035. doi: 10.1096/fj.201700384RR. Epub 2018 Jan 5.

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VCAM-1 expression is upregulated by CD34+/CD133+-stem cells derived from septic patients. PLoS One. 2018 Mar 30;13(3):e0195064. doi: 10.1371/journal.pone.0195064. eCollection 2018.

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Carnosine Catalyzes the Formation of the Oligo/Polymeric Products of Methylglyoxal. Cell Physiol Biochem. 2018 Mar 29;46(2):713-726. doi: 10.1159/000488727. [Epub ahead of print]

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Making room for new memories. Science. 2018 Mar 30;359(6383):1461-1462. doi: 10.1126/science.aat1493.

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Bringing European physiologists together. Acta Physiol (Oxf). 2018 Apr;222(4):e13043. doi: 10.1111/apha.13043. Epub 2018 Feb 15.

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Endothelial progenitor cells accelerate endothelial regeneration in an in vitro model of Shigatoxin-2a-induced injury via soluble growth factors. Am J Physiol Renal Physiol. 2018 Mar 7. doi: 10.1152/ajprenal.00633.2017. [Epub ahead of print]

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Astrocytic glutamine synthetase is expressed in the neuronal somatic layers and down-regulated proportionally to neuronal loss in the human epileptic hippocampus. Glia. 2018 May;66(5):920-933. doi: 10.1002/glia.23292. Epub 2018 Jan 19.

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Expansion of functional personalized cells with specific transgene combinations. Nat Commun. 2018 Mar 8;9(1):994. doi: 10.1038/s41467-018-03408-4.

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Modulation of glutathione peroxidase activity by age-dependent carbonylation in glomeruli of diabetic mice. J Diabetes Complications. 2018 Feb;32(2):130-138. doi: 10.1016/j.jdiacomp.2017.11.007. Epub 2017 Nov 22.


Institute of
Physiology and Pathophysiology

Heidelberg University

Im Neuenheimer Feld 326

69120 Heidelberg

Germany

Phone:+49 6221 54-4035
Fax:+49 6221 54-4038
E-mail:sekretariat.hecker@
physiologie.uni-heidelberg.de